But, in the spirit of the year, I would like to wish any and all who read my too-infrequent blog posts a Merry Luxmas and Happy Microbial Holidays, as I do each year.
First, I used LB plates with 0.2% arabinose and drew a bit with E. coli DH5alpha containing the famous plasmid pGLO, as well as the red pigmented Serratia marcescens. I tried to balance a small handheld longwave UV light with background illumination. Fluorescence versus prodigiosin created a pleasing green and red theme for the holiday, I hope. The results match the season, even if we have not more than a slight dusting of snow in Tacoma.
Of course, I adore "painting" or "drawing" with bioluminescent bacteria. In particular, a particular strain of Photobacterium leiognathi originally isolated by Eric Stabb and Ned Ruby from Kaneohe Bay, Hawai'i, remains a solid performer.
Here are some lovely "self-portraits" of bacterial bioluminescence. I like to experiment with reflections, as well. Regardless, I hope that these photos amuse and showcase my mania regarding True Microbial Supremacy!
My microbiology students this year even created a "Microbial Xmas Tree" powered by bacterial electricity!
Finally, no "Luxmas" celebration is complete without the video my wife Jennifer Quinn and I made a few years ago.
Happy Luxmas to all, and to each and every one of you, Merry Microbial Holidays!