Sunday, February 15, 2015

Happy Microbial Valentines Day---In Words of Living Light!

Well, time is flying, yet again!  I am beginning to get a better handle on my time, but still feel like I am swimming upstream.

But blog posts are not going to happen unless and post them.  There is a great deal that is going on for me professionally that I hope to announce soon, but in the meantime, here is a short post.

Valentines Day is fun for many people.  I certainly have enjoyed it a great deal over the years.  So why not mix it up with microbiology?

Most people who know me (or read this blog) are aware that I enjoy "painting" or "writing" with bioluminescent bacteria.  I then photograph the results in the dark.  For example, here is such an illustration featuring my undergraduate laboratory students.

I usually "paint" or "write" with Photobacterium leignothi, a bacterial strain originally isolated from Kaneoh'e Bay in Hawai'i by my friends and colleagues Eric Stabb and Ned Ruby.  It's very bright indeed, and perfect for this purpose.

So with that in mind, why NOT a "Living Valentines Day" card for my lovely and brilliant bride Dr. Jennifer J. Quinn of the University of Washington - Tacoma?

I even wrote a little Valentines Day poetry for her in living light.

But I also wanted to give a bioluminescent "shout out" to all of my microbiologist friends and colleagues.  So...

The entire process of light production by marine bacteria follows the density dependent process of quorum sensing (a concept that my poor students hear about endlessly from yours truly).  In any event, it occurred to me that the basic concepts of quorum sensing were worked out in luminous marine bacteria.  So why not write a "microbial haiku" about quorum sensing, appropriately illuminated by bioluminescence?

It's actually fairly challenging to write many words on Petri dishes with swabs (my normal way of carrying out this kind of task).   So I again tried a "Burma Shave" approach,  trying to write with toothpicks below.  It's not as even as I would like, but is another strategy.

Next time, sterile fine paint brushes?  If this keeps up, I will have to learn to paint as my wife does! 

In the meantime, I hope that everyone had a fabulous---even glowing---Valentines Day!

Wednesday, December 31, 2014

Do You Need Inspiration For 2015? Consider Randy Pausch's Advice.

Time does indeed move so very fast with every passing year.  We are coming up on 2015 as I write this post. When I was a boy, the year 2000 was a strange and exotic destination---I would be middle aged, and the world would be so very different.  Bright and shiny.  I thought we would have personal jet packs and spaceships to Mars. Oh well.  

At the same time, there are wonders all around us, if we but take the time to look and listen. Quiet contemplation is something from which we could all benefit, but we seem to have too little time for it, as a culture.

The approach of New Year's Day makes most people reflect a bit. And I was doing so, as the narcissist (and not in the self-congratulatory sense of the term) I suspect I am. I spent some time feeling unhappy with the things I had not done, the progress not met, the potential not met, the accomplishments not realized.  I felt and feel more than a little bitter at not reaching the dreams and goals in my heart. 

While it is true I am the luckiest person in the world from my perspective (to have the wife and children I do, the moderate health I enjoy, and a job that meets many of my professional needs), there is something in all of us that dreams of, as Edward G. Robinson put it in the move "Key Largo," more.

Then I remembered a hero of mine,  Randy Pausch.  The late Dr. Pausch was a charismatic computer scientist who chronicled his battle with pancreatic cancer and become something of a media icon before his death in 2008.  Pausch wrote a book called "The Last Lecture," which is both practical and highly inspirational. I have given away dozens of copies to undergraduate students.  If you have not read it, you should.  ASAP.

There are several quotes from Dr. Pausch's book that stick with me. I would like to share them with you.

This first one is so very important, especially to how I view science.
When you're eight or nine years old and you look at the TV set and men are landing on the moon---anything is possible.  And that is something we need to not lose sight of, is that inspiration and permission to dream is's important to have specific dreams.
I was that young boy watching Neil Armstrong on grainy television in 1969.  And many people, and many students, seem to be about settling, about limitations.  Focusing on what cannot be done, instead of working to...well, make their dreams come true.

Which leads me to:
Never lose the child-like wonder.  It's just too important. It's what drives us.
I have always loved science, even when it does not love me back. And I am awestruck by what is being discovered nearly every day. It's what makes me a scientist, even at my level.

Staying upbeat can be a challenge.  Dr. Pausch has it spot-on.
You just have to decide whether you are a Tigger or an Eeyore. You have to be clear where you stand on the Tigger/Eeyore debate.
I have spent too long as an Eeyore, when I am temperamentally meant to be a Tigger.  How about you?

Some wonderful advice about education.
The best way to teach somebody is to have think they are learning something else.  I've done it my whole career. And the head fake here is that they are learning to program, but they just think they are making movies and video games.
I have done that with my "extra credit creative projects" in my classes in recent years.  Pausch is absolutely right.

That in turn led me to:
The best gift an educator can give is to get someone to be self-reflective.
Yes! I have long said that the three most important words in science are "I don't know." Some folks don't agree, but I don't care. We can fix the "I don't know," and history has shown that inflexible and self assured attitudes harm science more than help it.  Certitude and authority are the enemies of science. It's how we know, as much as what we know.  Science is a process, not a destination.

And, more than a little painfully, Pausch shares some thoughts about succeeding in life (and academia).
General Advice:  (i) never break a promise, but renegotiate them if need be.  (ii)  If you haven't got time to do it right, you don't have time to do it wrong.  (iii) Recognize that most things are pass/fail.  (iv) Feedback loops:  ask in confidence.
Number three is particularly important.  We really do tend to think all life is a graded exercise.

Also, some stinging truths.
Experience is what you get when you didn't get what you wanted.
I realize that many, many people have had a more challenging path through science than I have. But I have had many, many reverses, poor choices, some bad luck, and, um, not so nice people with whom to contend. It can be frustrating.  It can make a person bitter, a sentiment with which I battle often.  And Randy Pausch's spirit has long chided me with this:
The brick walls are there for a reason.  The brick walls are not there to keep us out.  The brick walls are there to give us a chance to show how badly we want something. Because the brick walls are there to stop the people who don't want it badly enough.  They're there to stop other people.
I re-read this quote a lot when I am feeling low.

Here is one with which I have always struggled, courtesy of Dr. Pausch's high school football coach:

He said, when you're screwing up and nobody's saying anything to you anymore, that means they gave up.  And that's the lesson that stuck with me my whole life.  Is that when you you see yourself doing something badly and nobody's bothering to tell you anymore, that's a very bad place to be.  Your critics are the ones telling you they still love and care.
I think that entirely depends on the critics, but there is much truth in what Pausch's football coach said.

When dealing with difficult people, Dr. Pausch is very wise indeed.
Find the best in might have to wait a long time, sometimes years, but people will show you their good side.  Just keep waiting no matter how long it takes.  No one is all evil. Everybody has a good side.  Just keep waiting.  It will come out.
Dr. Pausch learned that from his high school football coach, again. The coach put it a bit differently.
When you are pissed off at somebody, and you're angry at them, you just haven't given them enough time.  Just give them a little more time---and they'll almost always impress you.
That one is particularly tough for me, given my path through academia, but it is also true.

Here is Pausch's quote from the end of his talk and his book that impacts me the most, emotionally:
Did you figure out the head fake? It's not about how to achieve your dreams.  It's about how to lead your life.  If you lead your life the right way, the karma will take care of itself.  The dreams will come to you. 
Did you figure out the second head fake? This talk's not for you. It's for my kids. 
Always makes me tear up. But then, I am a Sensitive New Age Guy™ in most ways.

There is much wisdom in his book.  Again, I highly recommend it.

But do read this book, and watch the videos.  You will be intellectually and ethically the richer for it, I promise.

For New Year's Day, may I suggest you watch his "last lecture"? I can promise you will find much of value in that 70-some-odd minutes.

His home page has many more resources and videos, all of which are uplifting and moving.

Let me go further, and recommend just a couple of more videos. Such a remarkable man, such a great loss. But look at how he was willing to share with all of us (and his family) quite a bit of wisdom and humor and bravery.

His widow Jai gives some thoughts about Pausch, his bravery, and the need for support into finding treatment or a cure for pancreatic cancer.

I think that Dr. Pausch's commencement speech at Carnegie-Mellon (not long before he passed away) is required viewing.

At the end of his speech, you will see Randy Pausch pick up his wife Jai to paradoxically show his strength (he was more than a bit of a showboat). She buries her face in his shoulder. I have read that she then whispered to him, "Don't you die on me" through tears, and he whispered back "I'm working on it."

Some of his dreams may seem trivial.  All of our dreams might be seen as such, from outside.  But they are our dreams.  

Randy Pausch loved "Star Trek," and had worked on some video games for that franchise.  William Shatner knew him slightly, and when he heard of Pausch's battle against pancreatic cancer, he characteristically sent a 8 x 10 head shot of himself (Mr. Shatner is nothing if not, um, self-assured).  But what Shatner wrote is moving.  "I don't believe in the no-win scenario," which is both a trope from "Star Trek," and a wonderful sentiment for Dr. Pausch.

Finally, when J.J. Abrams "re-booted" "Star Trek," he gave Randy Pausch a chance to have a bit part in the movie.  Here he is, though you need to look quickly:

So even one's most unlikely dreams can come true.

There is a poem I am fond of, by Delmore Schwartz, titled "Calmly We Walk Through This April's Day."  It has a line that always made me sad:
"...Time is the fire in which we burn..."
I first learned about this poem, in all places, from a "Star Trek" movie:  "Star Trek Generations."  It was uttered by a character trying to beat mortality in an unusual fashion (Malcolm McDowell is a remarkable actor, by the way). 

But the poem is much better than that.  The link above will take you to entire text, which is decidedly worth your time.

Notice the line I hope we can all (me emphatically included) recall more than the one that made me sad:
"...Time is the school in which we learn...."
Grab life as Randy Pausch suggested.  Make your dreams come true.  And as both Dr. Pausch and Winston Churchill urged:  never give up.

When my mother Wanda Martin was first diagnosed with ovarian cancer, I bought her this as a paperweight.

My father gave it to me the day after she passed away.  I look at it every day, and we should all take the lesson to heart.

What are your childhood dreams?  And what are you doing in 2015 to make them happen?

May 2015 make your dreams come true.  As for me, I have work to do.

Happy New Year to all!

Tuesday, December 23, 2014

Merry Luxmas and Happy Microbial Holidays!

That was a busy semester!  As the saying goes, "time doesn't fly; it flees!"  Teaching my beloved microbiology here at the University of Puget Sound, as well as a freshman writing seminar on symbioses, kept me far too busy.  I will be posting on both of these classes, which were utterly fabulous and filled with OMG™(Overwhelming Microbial Goodness) and SOS™(Superb and Outstanding Symbioses), in the coming weeks.  I will also be planning on improvements to my freshman biology course "The Unity of Life" (Biology 111, or an introduction to cell and molecular biology) to be taught next semester.  Whew.

But, in the spirit of the year, I would like to wish any and all who read my too-infrequent blog posts a Merry Luxmas and Happy Microbial Holidays, as I do each year.

First, I used LB plates with 0.2% arabinose and drew a bit with E. coli DH5alpha containing the famous plasmid pGLO, as well as the red pigmented Serratia marcescens. I tried to balance a small handheld longwave UV light with background illumination. Fluorescence versus prodigiosin created a pleasing green and red theme for the holiday, I hope.  The results match the season, even if we have not more than a slight dusting of snow in Tacoma.

Of course, I adore "painting" or "drawing" with bioluminescent bacteria.  In particular, a particular strain of Photobacterium leiognathi originally isolated by Eric Stabb and Ned Ruby from Kaneohe Bay, Hawai'i, remains a solid performer.

Here are some lovely "self-portraits" of bacterial bioluminescence. I like to experiment with reflections, as well.  Regardless, I hope that these photos amuse and showcase my mania regarding True Microbial Supremacy!

My microbiology students this year even created a "Microbial Xmas Tree" powered by bacterial electricity!

Finally, no "Luxmas" celebration is complete without the video my wife Jennifer Quinn and I made a few years ago.

Happy Luxmas to all, and to each and every one of you, Merry Microbial Holidays!

Friday, September 26, 2014

My First Radio Interview About Teaching and Microbial Supremacy!

I am thankful for any readers of this blog, but have always wanted to reach out more, to discuss Microbial Supremacy, Overwhelming Microbial Goodness (OMG), and Matters Microbial in general.  Someday, I would like to write a book.  Any guesses as to the title I am thinking of? Pretty obvious, really.

On campus, I am thought to be a little monomaniacal on the subject  (okay, a lot; guilty as charged).  But it was a fairly local phenomenon, with job interviews in the old days, a campus talk, and few presentations to interested parents.  Once I was able to do a little preaching of the One True Microbial Faith at the American Society of Microbiology General Meeting in San Francisco...but that was sadly a few years ago now.

Recently I was asked to be interviewed by the great folks at People Behind the Science.  Dr. Marie McNeely had a very nice discussion with me in late July, and that interview appears here. Here is another link:

I had a lot of fun, and readers will hear some stories I have related on this blog, such as how I became dubbed a "Microbial Supremacist," and my Richard Feynman story about how to find the right direction to pursue in science.

In all, I was able to talk a little bit about my history, about teaching, about science, and other things about which I am quite passionate. Many students I see are willing to "settle," not strive; I hope to encourage them to have a farther intellectual reach, and a richer life. 

People Behind the Science is a great podcast, with some truly fabulous interviews (I will not judge my own effectiveness). Scientists from everywhere in science talk about what motivates and excites them; their enthusiasm is concrete and memorable, in podcast after podcast.  

I highly recommend you take a listen---it's free!

Monday, September 8, 2014

Sometimes It Takes a Death to Teach Me About the Depths of Others...

I just received some sad news, so here is a short post to express how I feel about it.  As the poem by Delmore Schwartz, "Calmly We Walk through This April's Day,"  goes:

"Time is the fire in which we burn..."

But it goes on to add:

"Time is the school in which we learn...."

While I was in graduate school, one of the professors I saw around the building was Dr. Robert Schimke. He liked to tease and carry on, and I admit I didn't know him all that well. I kept my head low much of the time, and he was nothing if not outspoken.  So I sort of kept out of his way.  My 20s were a complicated time for me, and I often look back and wince. To those of you who knew me then, I can only say, "I'm sorry."  And I did eventually grow up (taking us back to the Delmore Schwartz poem).

Here is a photograph of the Bob Schimke I well remember from Stanford.


But here----is Bob Schimke the artist.

And I never knew about that aspect of his life.  I shake my head at that.

Anyway,  Bob Schimke married someone I quite adored, Dr.Patricia Jones (who was unfailingly kind and supportive toward me during my rather embarrassing "in my twenties" struggles in Palo Alto). So there had to be so very much that was good and wonderful about him, because of the undeniable good taste, ready humor,  and overall disposition of Pat Jones.

Robert Schimke, I have just heard, passed away. And in trying to learn more about him, I discovered quite a bit I did not know.  I didn't know those things while I was at Stanford, and I didn't know those things afterwards.  These were my losses, as it happens. I had heard Bob Schimke had had a serious accident, but didn't know how bad it was. It turns out that the accident was quite severe, but he used his situation in a positive way, making wonderful art. 

Read about it here, from 2012.  There is a great gallery of his art here at his Stanford website,  as well.  I kind of agree with what Bob Schimke said about the art of Jackson Pollock in his interview:   Bob's own work looks just about as good as Pollock's to me, too!  See what I mean?

And look at his stylist range in these two pieces (all images from his art website, here).

The first link above in the ASBMB Today piece is a wonderful interview and essay, and well worth your time to consider. What would you do, if you had to do something else than science? Or was it that Bob Schimke truly burned to be an artist all these years? But he did both, and was impressive in both areas.

How I wished I had known. I would have praised his artwork (not that the Bob Schimke I knew would care about praise, let alone praise from me!), and tried to purchase some of his work---because it is admirable art. Such depths to the man, and I never knew. It is true I have not been back to Stanford since I earned my PhD (I'm always a little embarrassed to be among the wonderful and accomplished people there, truth be told), but that isn't an excuse.  

The essay linked, and the artwork, remain a reminder.  We need remember that all of us have depths and talents that may not be apparent to people who don't take the time to really look---as I didn't look. 

So take a moment, and get to know the people around you. Look what I missed!  What other hidden talents and beautiful souls exist all around us, as we bumble our way through everyday life.  We should all take a deep breath, and really look at each other.  All of us have value, and admirable talents.

Some more than others, of course.  Rest in peace, Bob Schimke, and many condolences to his friends and family, in particular Pat Jones.

Monday, September 1, 2014

Catching Up, Part I: Meeting with Former Research Students at ASM in Boston in May.

You know, I keep meaning to post more often.  Then, as John Lennon famously observed, "Life is what happens when you are busy making plans."  So I am trying to post in a more regular fashion. Lots and lots to do, as is true for most of us; life is a juggling act. 

I have two courses this Fall semester:  one is my normal and much beloved Microbiology course (which I continue to want to call "Microbial Diversity," since I only get the one chance to promote Microbial Supremacy™ to students) for juniors and seniors, and a freshman "writing seminar" on Symbiosis and Parasitism (yes, the latter is part of the conceptual Venn diagram of the former, but many students don't yet know that).  So I expect to write quite a bit about these two courses as they unfold with the awesomosity I expect from our students here in Tacoma!

In the meantime, here is a post I meant to write a while back---I have such pride in my former research students, and I enjoy watching them develop their careers and lives.  I shine by their reflected glory!  

Anyway,  I was VERY happy that six of my former undergraduate students had the time to visit with me at the American Society for Microbiology General Meeting in Boston, last May.  I took them all out to dinner in Boston, and it was truly great to put them all at the same table, ranging across a lot of years, to swap stories and successes and challenges.  I have seldom felt prouder of my students, and more humbled by what I do for a living.

Here is the lineup:

From left to right, here are the former students, and what they are currently doing now.

  • Sarah Studer (Class of 2003, Occidental College).
  • Paula Welander (Class of 1998, Occidental College).
  • Desiree Baron (Class of 2001, Occidental College).
  • Andrew Collins (Class of 2007, University of Puget Sound) 
  • Morgan Giese (Class of 2014, University of Puget Sound) 
  • Kim Dill-McFarland (Class of 2011, University of Puget Sound)

Here is what each of them are currently up to:

Sarah is a science policy fellow in DOE's Fuel Cell Technologies Office, in the Office of Energy Efficiency and Renewable Energy (formal title:  EERE Postdoctoral Fellow). She provides expertise for biological hydrogen production projects in her division.  Sarah earned her PhD with my friend and MicrobialHero™ Ned Ruby at the University of Wisconsin Madison.  

Paula is an Assistant Professor of Environmental Earth System Science (and by courtesy, in Biology) at Stanford University. Her research team studies the biosynthesis and function(s) of molecular fossils (biomarkers) in bacteria. Paula earned her PhD with my colleague Bill Metcalf at the University of Illinois at Urbana-Champaign and did postdoctoral work with Dianne Newman at MIT (now back at Caltech).  Here is Paula's laboratory website.

Desiree is a Research Associate for Daryl Bosco at the University of Massachusetts Medical School, studying the interface between cell biology and amyotrophic lateral sclerosis. She earned her PhD with Kent Hill at UCLA, followed by postdoctoral work with Stephen Doxsey.  

Andrew is a fresh new postdoctoral fellow at the Forsyth Institute in Boston.  He will be working with Floyd Dewhirst, Gary Borisy, and Anne Tanner, studying the uncultured and unknown microbiota of the human mouth.  He earned his PhD with my friend Spencer Nyholm at the University of Connecticut Storrs.

Morgan is a fabulous worker looking for a biotech position in the Seattle area at present. Whoever hires her will be lucky indeed. Incidentally, here is a photograph of Morgan at her poster during the General Meeting.  I received some great reports about her professionalism while people chatted with her about her research...and I was not surprised.

Kim is a graduate student at the University of Wisconsin Madison, working on her PhD with Garret Suen.  Kim has worked on the microbiomes of a number of biological systems with Garret, ranging from hibernating squirrels to sloths (I'm told) to dairy cows.  

The dinner was a lot of fun, of course.  I had T-shirts made for my students (and it was hard to fit everyone into the photo, as you will see!), and gave everyone appropriate magnets to commemorate the event.  Here is the magnet.

I think the "Bdello Bdinner" is funny.  Adding "Bdoston" to may be over the top.  Oh, well.

This is the best I could get for a group shot in the T-shirts.  The restaurant was crowded.

Since Kim was left out of that shot, here is another photograph of her in her T-shirt. Please notice the FABULOUS mini-microfuge tube earrings.  They fluoresce under UV light, by the way.  Kim is stylish that way.

Embarassingly, I messed up some of the graduation dates on their T-shirts.  Sigh. Though I am often (as my father would put it) "a day late and a dollar short," my heart is in the right place.  Gulp!

A wonderful evening was had, and I am grateful to each and every one of these scientists for making some time to visit with me and bring me up to date.

By the way, I was delighted beyond words that two of my former undergraduate research students, Kim Dill-McFarland (mentioned above) and Jillian Waters (Class of 2008, University of Puget Sound) were at the most recent International Society for Microbial Ecology meetings in Seoul, Korea later last summer.  Here is the "twitter proof" that they were there together (courtesy of the very kind Kim).

After leaving the University of Puget Sound, Jill Waters earned her PhD at the University of Illinois at Urbana-Champaign (I have sent four undergraduate students there for PhDs so far!) with the late, great, Abigail Salyers (one of my professors at the Woods Hole Microbial Diversity Course, and a true MicrobialHero™ of mine), and is currently working on a postdoc with the quite fabulous Ruth Ley at Cornell.  Jill always wanted to be (her words) a "poop scientist."  

She is living the dream!

Anyway, there are lots and lots of days where I feel overwhelmed, buried, unsuccessful...fill in the negative blank.  But then I look at these great success stories.  While I think each and every one of them has earned every bit of their accomplishments through their brilliance and hard work, I like to think I had a bit of influence here and there.  Their reflected glory feels pretty nice.

It makes my workdays feel worthwhile, I must tell you.

Next year's ASM General Meeting is in New Orleans.  Perhaps there will be another Bdello Bdinner there, as well.  I certainly hope so!

I'm proud of you all!

Wednesday, July 30, 2014

Summer Research and Lab Themed Desserts!

I have long been super, super interested in bacterial predators like Bdellovibrio, which attacks and consumes a wide variety of Gram negative prey bacteria.  My first microbiology course at UCLA was taught by the late, great Syd Rittenberg, who was then one of THE authorities on the predator.  It's no wonder that the microbe always fascinated me.

Sadly, due to my own struggles in academia, and my position in the Trophic Web of Science at a small undergraduate institution (and without grants at present), I cannot make very big contributions to the field.  There are some true giants in "Bdellovibriology," like Liz Sockett, Edouard Jurkevitch, John Tudor, Henry Williams, Daniel Kadouri, and Eckhardt Strauch.  There are few others, though some friends of mine are starting in the field!

Bdellovibrio is not easy to work with on a daily basis, nor to unravel genetically, but I adore the wee beast.  I wrote a paper long ago about predatory microbes, and my lovely and brilliant bride Jennifer Quinn drew me the life cycle of the ravening microbe.

Many other more detailed or less stylistic depictions exist, but I like Jenny's.  There has always been, and remains, a great deal of mystery about Bdellovibrio's activities, which lead a former classroom student of mine at Occidental College to draw this great "Borg" view of the tiny predator!  Prey cells WILL be assimilated, after all.

When I took the life-changingt Marine Biological Laboratory's "Microbial Diversity" course at Woods Hole in 1996, the great microscopist Tom Pitta took some marvelous photomicrographs for me of Bdellovibrio attacking E. coli, seen here:

And a feeding frenzy by Bdellovibrio against E. coli here (cue the theme to "Jaws," please).

While I was in Los Angeles, I did some fascinating work with a fine scientist, Megan Nunez, who took some truly awesome atomic force micrographs of Bdellovibrio attacking a biofilm here.

And here at Puget Sound, an undergraduate student named Rob Chamberlain took this great electron micrograph of Bdellovibrio attacking Shewanella

Which brings us to today's festivities!  The Summer Research Program here at the University of Puget Sound is a great experience for our undergraduates.  They get a chance to really work hard on a research problem, without those pesky classes to get in the way. But research students need to blow off some steam from time to time.  One way is by fun events---in this case, our famous, delicious,  and totally fabulous "Lab Themed Dessert" competition.

And make no mistake, the competition is intense!  Students struggle to create tasty desserts reflecting their research projects or lab topics.  I have been fortunate in having Madison Cox in my laboratory, who is a great student, hard worker, and a fabulous baker.  Last summer, while she was working (as she does this summer) on the cloacal microbiota of the Striped Plateau Lizard (we have found some interesting aspects of these microbiota/communities, as seen here), Madison entered the Lab Themed Dessert competition for the first time.  

Madison won "Best Tasting" and "Most Aesthetically Pleasing" awards last summer for this masterpiece (note the swabs, inoculating loops, microbes and, um, back end of the lizard---as a cake).

This year, Madison decided to honor Bdellovibrio, by depicting the predator attacking and invading hapless E. coli!

A view to the front shows Madison's attention to anthropomorphic detail.

I got a kick out of watching Bdellovibrio invade the periplasm of the prey cell, as seen below.  The prey cell does not seen unhappy about this unfortunate process, I note. Note also the invading cell visible through the outer membrane of E. coli.

One of my other research students, Katie Frye, wrote up a nice summary of the inspiration for Madison's cakey creation. Collaboration abounds!

Madison did have an interesting approach to depicting the petite predator cells!  No, Bdellovibrio does not possess eyes nor teeth, but it is undeniably cute.

And here is my summer lab crew.  Clockwise from far left: Katie, Young Tia (our high school volunteer), Madison, Austin, and Cheyenne.

Since Tia doesn't like her photograph taken, we forced the issue with great enthusiasm, as you can see.  Never let your labcrew know your weaknesses.  Ever.  

Madison was very proud of her creation as she deftly dissected it for the crowd of ravenous summer research students.

It's well known that Bdellovibrio does not attack human cells.  But Madison seems not so sure.

Katie shares Madison's concerns.

Here is Madison receiving her "Most Creative" award from Dr. Leslie Saucedo in my department (in charge of the Summer Research Program).

Aaaannnddd...Madison coming right back up to receive her award for "Most Aesthetically Pleasing."  Well, Madison did kind of bodyslam the competition into submission with her entry.  Not that I'm prejudiced in favor Madison's artwork.  

Not at all.

Madison barely contains her glee at winning two of three awards, for the second year in a row.  She has skillz, friends.  Mad skillz.

And in the aftermath, three progeny Bdellovibrio cells surround a clay representation of the life cycle of Bdellovibrio created as a gift several years ago by a former student, Kat Schmidt.

And finally, Madison explains both Bdellovibrio and her culinary creation, in this YouTube video.  Thanks to Katie Frye for writing up the summary.

It was a great day for Madison.  For Bdellovibrio.  For Martin's Microbial Menagerie.  The winning entry was ambitious, delicious and predilicious!

Thanks for sharing with me a good day during Summer Research at the University of Puget Sound!