I have long been super, super interested in bacterial predators like Bdellovibrio, which attacks and consumes a wide variety of Gram negative prey bacteria. My first microbiology course at UCLA was taught by the late, great Syd Rittenberg, who was then one of THE authorities on the predator. It's no wonder that the microbe always fascinated me. Sadly, due to my own struggles in academia, and my position in the Trophic Web of Science at a small undergraduate institution (and without grants at present), I cannot make very big contributions to the field. There are some true giants in "Bdellovibriology," like Liz Sockett, Edouard Jurkevitch, John Tudor, Henry Williams, Daniel Kadouri, and Eckhardt Strauch. There are few others, though some friends of mine are starting in the field! Bdellovibrio is not easy to work with on a daily basis, nor to unravel genetically, but I adore the wee beast. I wrote a paper long ago about predatory microbes, and my lovely and brilliant bride Jennifer Quinn drew me the life cycle of the ravening microbe.
Many other more detailed or less stylistic depictions exist, but I like Jenny's. There has always been, and remains, a great deal of mystery about Bdellovibrio's activities, which lead a former classroom student of mine at Occidental College to draw this great "Borg" view of the tiny predator! Prey cells WILL be assimilated, after all.
When I took the life-changingt Marine Biological Laboratory's "Microbial Diversity" course at Woods Hole in 1996, the great microscopist Tom Pitta took some marvelous photomicrographs for me of Bdellovibrio attacking E. coli, seen here:
And a feeding frenzy by Bdellovibrio against E. coli here (cue the theme to "Jaws," please).
While I was in Los Angeles, I did some fascinating work with a fine scientist, Megan Nunez, who took some truly awesome atomic force micrographs of Bdellovibrio attacking a biofilm here.
And here at Puget Sound, an undergraduate student named Rob Chamberlain took this great electron micrograph of Bdellovibrio attacking Shewanella.
Which brings us to today's festivities! The Summer Research Program here at the University of Puget Sound is a great experience for our undergraduates. They get a chance to really work hard on a research problem, without those pesky classes to get in the way. But research students need to blow off some steam from time to time. One way is by fun events---in this case, our famous, delicious, and totally fabulous "Lab Themed Dessert" competition. And make no mistake, the competition is intense! Students struggle to create tasty desserts reflecting their research projects or lab topics. I have been fortunate in having Madison Cox in my laboratory, who is a great student, hard worker, and a fabulous baker. Last summer, while she was working (as she does this summer) on the cloacal microbiota of the Striped Plateau Lizard (we have found some interesting aspects of these microbiota/communities, as seen here), Madison entered the Lab Themed Dessert competition for the first time. Madison won "Best Tasting" and "Most Aesthetically Pleasing" awards last summer for this masterpiece (note the swabs, inoculating loops, microbes and, um, back end of the lizard---as a cake).
This year, Madison decided to honor Bdellovibrio, by depicting the predator attacking and invading hapless E. coli!
A view to the front shows Madison's attention to anthropomorphic detail.
I got a kick out of watching Bdellovibrio invade the periplasm of the prey cell, as seen below. The prey cell does not seen unhappy about this unfortunate process, I note. Note also the invading cell visible through the outer membrane of E. coli.
One of my other research students, Katie Frye, wrote up a nice summary of the inspiration for Madison's cakey creation. Collaboration abounds!
Madison did have an interesting approach to depicting the petite predator cells! No, Bdellovibrio does not possess eyes nor teeth, but it is undeniably cute.
And here is my summer lab crew. Clockwise from far left: Katie, Young Tia (our high school volunteer), Madison, Austin, and Cheyenne.
Since Tia doesn't like her photograph taken, we forced the issue with great enthusiasm, as you can see. Never let your labcrew know your weaknesses. Ever.
Madison was very proud of her creation as she deftly dissected it for the crowd of ravenous summer research students.
It's well known that Bdellovibrio does not attack human cells. But Madison seems not so sure.
Katie shares Madison's concerns.
Here is Madison receiving her "Most Creative" award from Dr. Leslie Saucedo in my department (in charge of the Summer Research Program).
Aaaannnddd...Madison coming right back up to receive her award for "Most Aesthetically Pleasing." Well, Madison did kind of bodyslam the competition into submission with her entry. Not that I'm prejudiced in favor Madison's artwork. Not at all.
Madison barely contains her glee at winning two of three awards, for the second year in a row. She has skillz, friends. Mad skillz.
And in the aftermath, three progeny Bdellovibrio cells surround a clay representation of the life cycle of Bdellovibrio created as a gift several years ago by a former student, Kat Schmidt.
And finally, Madison explains both Bdellovibrio and her culinary creation, in this YouTube video. Thanks to Katie Frye for writing up the summary.
It was a great day for Madison. For Bdellovibrio. For Martin's Microbial Menagerie. The winning entry was ambitious, delicious and predilicious! Thanks for sharing with me a good day during Summer Research at the University of Puget Sound!
There are many things I enjoy about teaching and doing research with undergraduates (and yes, there are challenges, too) here in the Biology Department at the University of Puget Sound. From time to time---always a pleasant surprise---a student will send me a "thank you" note after graduation. Such notes are very nice to receive, and to hold in reserve for those difficult times all academics face periodically. Maybe as a deposit to our virtual "I'm okay at my job" emotional bank account, for those days when we have doubts. By the way, isn't it interesting how we as a culture have no trouble sounding off when things are not to our liking...but seem to have problems telling others when they have done something well, or have had some kind of positive impact on our lives? Why is that? Anyway, meet a young lady named Ariana, a transfer student to the University of Puget Sound. She was assigned to my advisee caseload, and we worked together for a bit over two years. Ariana was always quiet and a little nervous. It was great to see her gain confidence over the next two years. She even did some research with a colleague of mine, so I was able to work with Ariana a bit in the lab last summer. Ariana graduated in May, and is off to a library science M.S. program in Chicago (she always did love libraries!). Ariana came to visit the lab this morning, and look at what she made me! It was a nice surprise, to say the least. Ariana poses with her knit bacteriophage here.
Also, the bacteriophage was happy to consume a donut at morning lab meeting. And yes, Ariana stitched my first name onto the knit face of that icosahedral head (which made some of my current students snicker that Ariana was implying that I am a virus). But heck, to misquote the Bard of Avon, "...all the world's a phage...," right?
Here is a nice photo of the impressive knitting project, with a hand for scale.
I don't know exactly what pattern Ariana used. This is a possibility. I'm currently thinking about use thick wire to stiffen the phage legs and body, so that I can find a place to hang up the item in my lab or office. This is why I have a job that is nearly perfect for me, right here. The surprises that let me know what I do and say matter to people mean more than I can easily express. Such a nice surprise! Thank you, Ariana. It was a pleasure working with you while you were a student at the University of Puget Sound. I really appreciate the gift, and the kind words. Good luck in Chicago!
Just a quick post, and kind of a somber one. Hopefully, it will have a bit of impact as the new academic year approaches, and get educators like myself thinking. Imagine a classroom of 100 freshmen students. Some eager, some nervous, some world-weary and cynical, and some half-asleep. In other words, a slice of the college age, university attending, population. Ask them if they know the name "Norman Borlaug." Then ask them if they know the name "Kim Kardashian." I think you can tell where I am going with this post. You haven't done it yet as I have, yet you can---each and every one of you---predict the responses. Kim Kardashian is an inexplicably well-known media celebrity. Come to think of it, she first reached the public eye in a somewhat tacky fashion(though she is far from the only celebrity to use that route to notoriety). She grabbed at her fame and has monetized it heavily, leveraging a career of sorts. Can you blame her? It's all about getting noticed. By the way, feel free to insert the name of any male celebrity if you wish. But Norman Borlaug is a name everyone should know. I don't want to go over his accomplishments. What I want is anyone reading this post who doesn't know about Borlaug's life and work to read the first link I posted. Then watch Penn and Teller talk about Borlaug here. Read this. Then go on to read this. Or even watch the whole documentary on Borlaug here. I don't know many people who spearheaded efforts to save a billion human beings from starvation, successfully. But he didn't get his own reality show, did he? Perhaps you want something more media-savvy and hip about Norman Borlaug. How about this?
Isn't that a fantastic way to publicize what Borlaug did? Did you know all that about Norman Borlaug? The only reason I did was from debating on scarce world resources in high school, and my plant science professors at UCLA. Tell your friends. And use Borlaug's great comment:
I'm not one to sit idly by...I'm going to play that card, and play it hard.
Indeed. I sent a friend of mine this "Scientist Rock Star" poster celebrating Borlaug recently.
I recommend you order a Borlaug poster from Megan Lee, and post it in your office or lab or classroom. Do the same with other scientists who need the PR. For example, this is something I have posted on my office door, not that it surprises anyone.
We need to have a world where we recognize people for their accomplishments. By the way, don't think that a Microbial Supremacist™ like me is going all plant-centric on you! Read this American Academy of Microbiology 2013 report, titled "How Microbes Can Feed the World," available here. The Small Masters are always in my thoughts. And brain, apparently. All of this discussion of media popularity versus significance reminds me of the quote by Oscar Wilde: "I would rather be infamous than not famous at all." That is how most of our celebrity culture works. But should it? We need to find ways to make things better, not "sit idly by" as Norman Borlaug put it. And when we find it, we need to "play that card, and play it hard." I'm with Penn Jillette in considering Norman Borlaug one of the greatest human beings in history---and saddened how few folks know his name. Penn puts it this way, here:
"Norman is the greatest human being in history, and you probably never heard of him."
Tell a friend today about Norman Borlaug, and other real heroes, rather than what's in the National Enquirer. Honor the people who labor anonymously to make things better for all of us. Play that card, readers, and play it hard.
You know, I keep meaning to post more often to this blog. But, as John Lennon famously observed, "Life is what happens to you while you're busy making other plans." So I will try to do better. I have several interesting posts cooking, to keep readers up to date. Hard to say how often people read these posts, but they have some value for me at least (in getting my thoughts down and events remembered). Thus, onward and upward... But today is the 4th of July, a mixture of holiday and history. Many Americans celebrate with fellowship, food, and fireworks. With the help of some of my summer research students, we created our own "fireworks" of a more biological nature, using our favorite bioluminescent bacterium, Photobacterium leignothi. Since I recently dropped and damaged my "regular" camera (a Canon G12), I am doing my best with my wife's camera. The more I think about the relationship between science and art, and how much I enjoy thinking about images (microbiological or otherwise), the more I think I need to upgrade my camera. Yes, yes, after I hit the lottery... Anyway, this is the best I could do this morning using the great plates my students "painted" with a bacterial culture yesterday afternoon.
Have a happy, safe, and restful 4th of July, everyone! That goes for your microbiota as well as your eukaryotic cells.
After all, we each and every one of us are "anthology" organisms, made up of a vast and various community of living things. But I hope that "all of you individually" agree with me that microbial supremacy is both wonderful and here to stay!
